Expediting Antibody Discovery with a Cell and Bead Multiplexed
Por um escritor misterioso
Last updated 07 julho 2024
![Expediting Antibody Discovery with a Cell and Bead Multiplexed](https://d3i71xaburhd42.cloudfront.net/6607b0810cbf8510cad934e8dcbace8076997a96/5-Figure2-1.png)
A high-throughput, multiplexed cell and bead-based competition assay that identifies and quantitates mouse IgG isotypes and assesses cell health is developed that can reduce the time to antibody cloning and production. With advances in molecular engineering and humanization, monoclonal antibodies are one of the fastest-growing classes of biopharmaceuticals. During antibody discovery, antibody from hybridoma or primary B-cell supernatants is screened for the desired binding characteristics, and secondary screens measure antibody function and concentration, identify immunoglobulin G (IgG) isotype, and assess cell health. In order to expedite the antibody discovery process, we developed a high-throughput, multiplexed cell and bead-based competition assay that identifies and quantitates mouse IgG isotypes and assesses cell health. No differences in assay performance were observed between single and multiplex formats. The linear range of the assay was from 0.5 to 50 µg/mL, and washing was not required, decreasing assay time and variability. Slight modifications to the protocol allowed quantification of dilute antibody supernatants (0.1–5 µg/mL). Using hybridoma cultures, we showed that cell viability measurements in the assay did not interfere with the bead-based IgG measurements. The assay described here is a simple mix-and-read, no-dilution screen that can reduce the time to antibody cloning and production. The high-content data can differentiate monoclonal and polyclonal wells, determine IgG quantity for downstream functional assays, provide isotype information, and monitor cell proliferation and viability.
![Expediting Antibody Discovery with a Cell and Bead Multiplexed](https://iiif.elifesciences.org/lax/67106%2Felife-67106-fig4-v2.tif/full/,1500/0/default.jpg)
Human immunocompetent Organ-on-Chip platforms allow safety
![Expediting Antibody Discovery with a Cell and Bead Multiplexed](https://www.researchgate.net/publication/321247646/figure/fig2/AS:673301517053976@1537538999686/Characterization-of-antibodies-from-the-established-hybridomas-by-the-MIHS-method-A.jpg)
Characterization of antibodies from the established hybridomas by
![Expediting Antibody Discovery with a Cell and Bead Multiplexed](https://resources.rndsystems.com/images/datasheets/lxsapm_porcine-luminex-discovery-assay-9620211173.png)
Porcine Luminex® Discovery Assay (LXSAPM): R&D Systems
![Expediting Antibody Discovery with a Cell and Bead Multiplexed](https://d2jx2rerrg6sh3.cloudfront.net/image-handler/picture/2018/6/Intellicyt_Art3_Pic2.jpg)
Using Multiplexed Screening for Advanced Therapeutic Antibody
![Expediting Antibody Discovery with a Cell and Bead Multiplexed](https://pubs.rsc.org/image/article/2021/LC/d1lc00243k/d1lc00243k-f5_hi-res.gif)
Miniaturized single-cell technologies for monoclonal antibody
![Expediting Antibody Discovery with a Cell and Bead Multiplexed](https://journals.sagepub.com/cms/10.1177/JBXB_23_7/asset/164b79bc-c416-b79b-8c41-4b79bc8c4164/jbxb_23_7.largecover.png)
Expediting Antibody Discovery with a Cell and Bead Multiplexed
![Expediting Antibody Discovery with a Cell and Bead Multiplexed](https://www.bioradiations.com/wp-content/uploads/2018/07/18-0586-banner.jpg)
Multiplex Cytokine Immunoassays
![Expediting Antibody Discovery with a Cell and Bead Multiplexed](https://www.bioradiations.com/wp-content/uploads/2020/12/20-0744-figure.jpg)
Multiplex Immunoassays in the COVID-19 Vaccine Race
![Expediting Antibody Discovery with a Cell and Bead Multiplexed](https://www.bio-rad.com/sites/default/files/2022-09/ci_hivag-ab_multiplex_1.png)
HIV Ag-Ab Multiplex Panel
Ultrasensitive Multiplex Imaging of Cell Surface Proteins via Core
![Expediting Antibody Discovery with a Cell and Bead Multiplexed](https://www.genengnews.com/wp-content/uploads/2023/03/Cover-image_Antibody-3D-Rendering-QBeads-Webpage-Abobe-Stock-212148709-en-Sartorius-1-scaled.jpeg)
Expediting Antibody Discovery and Characterization with Flow Cytometry
![Expediting Antibody Discovery with a Cell and Bead Multiplexed](https://pubs.acs.org/cms/10.1021/acssensors.2c01750/asset/images/medium/se2c01750_0007.gif)
Extracellular Vesicle Antibody Microarray for Multiplexed Inner
![Expediting Antibody Discovery with a Cell and Bead Multiplexed](https://ars.els-cdn.com/content/image/1-s2.0-S2472555222068939-gr3.jpg)
Expediting Antibody Discovery with a Cell and Bead Multiplexed
![Expediting Antibody Discovery with a Cell and Bead Multiplexed](https://d3i71xaburhd42.cloudfront.net/6607b0810cbf8510cad934e8dcbace8076997a96/6-Figure3-1.png)
Expediting Antibody Discovery with a Cell and Bead Multiplexed
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